Faculty of Science - Leading creativity and innovation in the sciences


Data Processing and Analysis


Understanding and presenting your data


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The raw data output needs to be processed before presentation.  The more complex the data, the more sophisticated the analytical strategies required.

Establishing solid identifications, relative abundance and statistical analysis to identify significant differences between study groups, constitutes the first step in this process.

Next, all identified proteins and / or metabolites are grouped according to their known functions and the proteomic / metabolomics profile analysed as a whole in terms of biological process or cellular structure. A literature search is performed to piece together the investigation and cutting edge software is used for the pathway analysis.

A typical proteomics project will generate data from tens of thousands of unique peptides representing 3000 - 6000 proteins.  The processing of such large data files is not for the faint hearted and is often delegated to experts.

 

This service includes:

  • Advice on isobaric labelling schemes prior to proteomic analysis (iTRAQ)
  • Data processing from mass spectrometry output to final identifications, relative abundance and statistical analysis.
  • Compilation of results tables with summary statistics
  • Complete pathway analysis
  • Written report detailing methods, key findings and suggestions for visual presentation.

 

 

Visualisation


 

Large throughput techniques generate masses of data that can be difficult to communicate efficiently. Including an eye-catching figure outlining the key findings can greatly enhance the impact of a publication.

Dr. Mia Jüllig is a senior research fellow in the School of Biological Sciences. She specializes in the visualization of biochemical concepts and key findings arising from complex datasets. This includes metabolic pathways, protein clusters and procedural flow schemes, as well as more imaginative designs for graphical abstracts and front covers.

She can also produce illustrations for presentations and teaching. 

 

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Please contact Mia for more information or assistance.