The 3130XL is a DNA sequencing machine that analyses DNA samples via sanger sequencing (determines the sequence of specific DNA) and genotyping (for sizing of specific DNA fragments) using capillary electrophoresis coupled with fluorescent dye technology.
The DNA sequencing centre offers the 3130XL as a service to UoA and external clients with a number of different options available.
Sanger sequencing options available:
Full sequencing service:
The Facility undertakes both the fluorescent chemical reaction and electrophoresis run.
Capillary electrophoresis service:
The client does the fluorescent chemical reaction and the Facility runs the electrophoresis.
Genotyping options available:
Genotyping Internal lane standard size ladder options:
Rox (Red dye) ladder 400HD / 500HD
For the dye selection combination 6 fam/hex/ned.
Liz (Orange dye) ladder 600 /1200
For the dye selection combination 6 fam/vic/ned/pet.