Faculty of Science - Leading creativity and innovation in the sciences


MicroCT and X-ray microscope


Auckland Bioengineering Institute

Instructions for using and requesting services of the MicroCT and X-Ray microscopes


All users must first register with ASAS-iLab

Registered users can directly Login to ASAS-iLab

Via ASAS-iLab, a user can submit a project request to be evaluated by the technical manager. This will involve reviewing the scope of the project, provide an estimation of feasibility, cost and time required.

What is MicroCT and X-Ray microscopy?


MicroCT is a high resolution X-ray computed tomography. It results in a three dimensional image of X-ray attenuation throughout the sample.

In most cases samples for microCT require no special preparation as contrast is obtained due to the differing absorption of X-rays.  However for soft tissue biological samples staining is required and there are simple methods available.

Magnification is achieved geometrically by the relationship of the sample & camera. Resolution and field of view depend on each other as well as being sample size dependent. Thus imaging at high magnification (0.5 µm pixels) has a field of view of 2.4 x 1.6 mm and a sample diameter of less than 2 mm.

MicroCT can also be used as an X-ray microscope to quickly obtain 2D structural and density differences in thin samples.

MicroCT is not a quick technique as the acquisition of thousands of individual projections can take hours per sample and is highly dependent on spatial resolution requirements. It is data and computationally intensive thus requiring Tb of storage and high end computing hardware.

MicroCT is capable of dynamic experiments using tension / compression and cooling / heating stages.

An operator is available to run samples and training can be given to users who have long term requirements.

There are fees associated with use of the instruments, services offered and operator time.

MicroCT videos of rat heart and porcine skin injection
MicroCT videos of wet clay and apple flower

SkyScan 1272 desk top X-Ray microtomograph


call-to-action-science-book-now
1272 Pic

New generation instrument capable of sub-micron pixel resolution

Microfocus sealed X-ray source 20-100 kV, 10W, <5 µm spot size @4W

X-Ray detector 16Mp (4,904 x 3,280 pixels) 14 bit cooled CCD fibre-optically coupled to scintillator.

Pixel size on the object at maximum magnification (nominal resolution) is 0.35 µm (4,904 x 3,290 pixels field of view 1.7 x 1.2 mm) or reconstruction arrays - up to 14,450 x 14,450 x 2,630 pixels (16Mp) after a single scan.

Maximum scanning object diameter is 75 mm using three camera offset positions, scanning diameter 52 mm using two camera offset positions, scanning diameter for fastest scan (central camera position) is 26 mm.

6 Position integrated filter changer, standard filters: no filter, Al 0.25 mm, Al 0.5 mm Al 1 mm Al 0.5 mm + Cu 0.038 mm, Cu 0.11 mm.

Integrated micro-positioning stage, 5 - 7 mm travel (dependent on orientation).

Radiation safety: < 1 microSv/h in 15 cm from any point of instrument surface.

 

 

SkyScan 1172 desk-top X-Ray microtomograph


call-to-action-science-book-now
1172

Recently refurbished with new camera and X-ray source

Microfocus sealed X-ray source 2--100 kV, 10W, <5 µm spot size @ 4W.

X-ray detector 11Mp (4,000 x 2,664 pixels) 14 bit cooled CCD fibre-optically coupled to scintillator.

Pixel size on the object at maximum magnification is 0.5 µm (4,000 x 2,664 pixels). Field of view is 2 mm x 1.4 mm.   

Maximum scanning object diameter is 75 mm using two camera offset positions, scanning diameter 52 mm using two camera offset positions, scanning diameter for fastest scan (central camera postion) is 26 mm.

3-Position integrated filter changer, standard filters: no filter, Al 1 mm, Al 0.5 mm + Cu 0.08 mm.

Radiation safety: < 1 microSv/hr in 15 cm from any point of instrument surface.

Enquiries

For academic project proposals contact Prof. P Hunter - Director. 

For operational matters and technical enquiries contact Dane Gerneke - Manager

Location

Ground floor (south side), 70 Symonds Street, Auckland 1010. NZ

Core hours of operation: 9 am to 5 pm

Bookings:

Approved users may book the instrument directly.

All others and those who require the presence of the technical manager must contact Dane who will then advise them when time is available.

 

 

 

Services offered

 

New Samples One time cost at the outset of the project for protocol development charged per hour
Instrument time Instrument time is charged per operator hour
Reconstruction Computational image reconstruction is charged per operator hour
Image Processing, 3D visualisation and making of movies Charged per operator hour
Image analysis Analysis of reconstructed data sets charged per operator hour
Written report request Charged per operator hour
Software Analytical software is available at no charge to users

Hourly rates are available upon logging in to ASAS-iLabs