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Auckland Genomics


The University of Auckland Genomics Centre offers a range of Illumina sequencing platforms (MiSeq, NextSeq), as well as access, via our network, to HiSeq, X10 and PacBio.

A full ABI Sanger sequencing and genotyping service is also available through the DNA Sequencing Centre.
 

Bioinformatics services

Our bioinformatics team is available to discuss scientific collaborations on a case-by-case basis.

In addition, we can provide the following bioinformatics service and support:

  • Advice on experimental design as it pertains to downstream bioinformatics analyses
  • Post-production QC of sequencing and array data
  • Assistance with genome/transcriptome assembly/mapping
  • 16S/COI OTU calling
  • Setup and running of custom/batch BLAST searches
  • Educational training, upskilling & custom workshops

 

Platform Illumina MiSeq
Application

Metagenomics and amplicon sequencing

Bacterial whole genome sequencing

Small RNA sequencing

Length of Reads 2 x 25 bp to 2 x 300 bp
Number of Reads Up to 20 - 30 million paired end reads / run

Illumina Sequencing


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The Illumina MiSeq is a next generation desktop DNA sequencer using industry standard genomics workflows, with high accuracy, sensitivity and specificity.

The Genomics Centre offers inhouse Illumina MiSeq runs, and access to HiSeq, X10 and PacBio sequencing.

Services

  • We can prepare all standard library types, for sequencing inhouse, or on other platforms via our collaborators, including:
  • DNA libraries, both low and standard input
  • Small RNA Libraries
  • Amplicon Indexing/Metagenomic Libraries
  • Nextera/Low Input Nextera Libraries
  • Bisulfite Libraries
  • Targeted Resequencing
  • Illumina VeriSeq Embryonic Preimplantation Genetic Screening
  • Small Genome Resequencing
  • Single Cell RNAseq (late 2017)
  • mRNA Sequencing – including molecular indexing
     

Genomic DNA Extraction, QC and submission for X10 or PacBio

We can assist researchers in the production of high-molecular weight DNA required for long reads and other whole genome sequencing applications. We can provide QC of DNA, complete the sample submission process and arrange shipping/customs clearance.

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Affymetrix GeneChip System


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The Affymetrix GeneChip® Instrument System is a fully integrated platform for conducting gene expression research using GeneChip® brand probe arrays. 

Affymetrix experiments can be very quick to design and complete. The data can also be analysed extremely rapidly, yielding widely understood and verifiable data to speed experimental progress.

Our Services include

  • The Affymetrix GeneChip® Instrument System offers cost-efficient gene expression analysis per sample
  • Due to the simplicity of analysis, projects can attract lower bioinformatics costs than RNAseq experiments.

Techniques

  • RNA or DNA samples can be submitted for gene/miRNA expression analysis or SNP detection, on GeneChip® brand probe arrays.
  • We offer a variety of labelling methods for gene expression using either 3’ or whole transcript labelling.
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Contact


Contact: genomics@auckland.ac.nz | 09 923 7653