The PGM is a desktop next generation DNA sequencer which harnesses the power of the Life Technologies AmpliSeq oligo design website and database to create pools of primers capable of large targeted resequencing projects, creating ready-made libraries for sequencing on the PGM.
The PGM is used for sequencing PCR amplicons at low cost and with high speed turnaround. PCR amplicon samples produced using predesigned or custom primer panels can be submitted for sequencing on the PGM.
- PGM reagents enable a choice of chips (400,000 to 5,000,000 reads), and up to 400 bp read lengths
The following pre-designed panels are single pools of primers used to target and sequence genomic ‘hotspot’ regions. Lists of targeted genes and mutations are available from the Life Technologies website.
Ion AmpliSeq™ Cancer Hotspot Panel v2
Ion AmpliSeq™ Comprehensive Cancer Panel
Ion AmpliSeq™ Inherited Disease Panel
Ion AmpliSeq™ Sample ID Panel
Ion AmpliSeq™ Community Panels Flyer
Ion AmpliSeq™ Colon and Lung Panel Flyer
Ion AmpliSeq™ BRCA1 and BRCA2 Panel Flyer
Custom amplicon libraries can also be easily designed using the AmpliSeq website to target other regions of interest.
- When used in conjunction with Ion Reporter Software, the PGM allows the rapid and cost effective detection of sequence variation in PCR amplicons.